Safety and Stability of Intra-stromal Implantation of Human Amniotic Membrane
Hossein Aghamollaei1 *, Khosrow Jadidi1 , Farshid Karimi1 , Setareh Besharatzad2
- Chemicals Injures Research Center, Systems Biology and Poisonings Institute, Baqiyqttallah University of Medical Sciences, Tehran Iran
- Vision Health Research Center, Semnan University of Medical Sciences, Semnan, Iran
Abstract: Amniotic membrane has wide application in treatment ocular surface disorders. The stability of this natural scaffold in the stroma has not reported yet. The aim of this study was to investigation of safety and stability of amniotic membrane implantation in the rabbit’s corneal stroma.
Methods: Six male New Zealand white rabbits were anaesthetized and a 3 mm pocket was created at the mid stroma. Human decellularized amniotic membrane was implanted in the pocket. Slit lamp examination was performed for all rabbit’s eyes and any sign was recorded. Rabbits were euthanized after 1 month (n=3) and 3 months (n=3) and cornea were removed to evaluation of histological finding.
Results: No clinical rejection was detected during the study. The eyes were normal without any opacity, tearing, neovascularization, swelling or injection. Histological evaluation showed that amniotic membrane was integrated into the stroma without any adverse effect on surrounding tissue.
Conclusion: Intra stromal implantation of decellularized amniotic membrane is a safe method for corneal tissue engineering. This scaffold is stable for at least 3 months in the stroma.
