Evaluation of candidate cytokines in conjunctival-exposure to plasma in rat tear and sera
Khosrow Jadidi1 *, Farhad Nejat1 , Shima Eghtedari2 , Seyede Yasamin Adnani2 , Shiva Pirhadi2
- Vision Health Research Center, Semnan University of Medical Sciences, Semnan, Iran
- Vision Health Research Center, Semnan University of Medical Sciences, Semnan, Iran;
Abstract: Atmospheric Low-Temperature Plasma (ALTP) has been proved its efficacy in eye surgery, especially in conjunctival cyst ablation, but little is known about how ALTP modulates inflammatory responses.
Methods: Herein, we evaluated the effects of ALTP-exposure on conjunctiva tissue on the inflammatory responses. To this end, we used 8 female adult Lewis rats that were followed-up as 4 groups. While the right eye of each rat was chosen for the test, the left eye was considered as a control. The plasma was generated by the white handpiece of the plasma generator device and 3 spots applied on conjunctiva. To follow-up the early clinical outcomes after plasma exposure, the slit-lamp biomicroscope examination was performed. Tear and serum samples were isolated from each rat and the concentration of pro-inflammatory (i.e. IL-1α, IL-2, IL-6, IFN-γ, and TNF-α) and anti-inflammatory cytokines (i.e. IL-4 and IL-10) was measured using flow cytometry
Results: The external and slit-lamp examinations showed affection after 48 hours ,but no ocular surface complications after 6 month follow up in ALTP-exposed rats. The analysis revealed that the ALTP transiently increases the concentration of pro-inflammatory cytokines—IL-1? and IL-2 in tear samples; no differences were observed regarding other pro- and anti-inflammatory cytokines in the rat tear or serum samples after 6 month follow up .
Conclusion: ALTP probably can be used as an alternative therapeutic method for some ocular surface disorders; This technique demonstrated no stable inflammatory responses than other conventional treatment procedures. Axiomatically, this can keep the patients from consuming a long time prescribed drugs for mitigating the inflammation after surgery.
