Effects of the Secretome of Human Wharton's Jelly Mesenchymal Stem Cells on the Proliferation of the Retinal Pigmented Epithelium
Fatemeh SanieJahromi1 , M. Hossein Nowroozzadeh1 , Zahra Khodabandeh2 , Zahra-Soheila Soheili3 , Zohreh Khajehahmadi1 , Zahra Emadi1 , Mohammad Reza Talebnejad1 *
- Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
- Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran
- National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract: Human retinal pigmented epithelium (RPE) can undergo an uncontrolled proliferation in some disorders such as retinal detachment associated with proliferative vitreoretinopathy (PVR). The present study was conducted to evaluate the effect of the conditioned medium secreted by human Wharton's jelly mesenchymal stem cells (WJMSCs-CM) on the proliferation of the RPE.
Methods: WJMSCs-CM was collected from WJMSCs after two periods of 24-h and 9-h culture in serum-free medium. RPE cells were cultured in WJMSCs-CM versus serum-deprived media for 24 h. The effect of WJMSCs-CM on RPE cell proliferation was determined using the MTT assay.
Results: MTT assay demonstrated that RPE cell viability was reduced significantly in WJMSCs-CM treated RPE cells compared to those cultured in serum-deprived medium (64.23 ± 2.44 vs 100.10 ± 5.68; P = 0.006).
Conclusion: The represented data showed that WJMSCs-CM could significantly reduce the proliferation of RPE cells in vitro. This proof-of-the-concept study provides basic evidence for the possible effect of WJMSCs-CM on preventing PVR. However, it is still a long way to apply this modality in humans.
